Digoxigenin 抗体
This anti-Digoxigenin antibody is a 绵羊 多克隆 antibody detecting Digoxigenin in WB, IHC (fro) 和 EIA. Suitable for Digitalis purpurea (Common foxglove) 和 Digitalis spec..
Quick Overview for Digoxigenin 抗体 (ABIN113360)
抗原
Digoxigenin (DIG)
适用
Digitalis purpurea (Common foxglove), Digitalis spec.
宿主
绵羊
克隆类型
多克隆
标记
This Digoxigenin antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
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纯化方法
- Affinity Chromatography on Protein G .
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免疫原
- Digoxin-3-CMO conjugated to KLH
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应用备注
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Immunohistochemistry on Frozen Tissue. Western blot. ELISA (1/100-1/5,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 5.0 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Digoxigenin (DIG)
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别名
- Digoxigenin
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物质类
- Chemical
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背景
- Digoxigenin is a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production. Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
抗原
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